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Fluorescent Dyes  >  Reactive Fluorescent Dyes  >>  5-IAF

Product Name 5 - IAF
[5 - Iodoacetamidofluorescein]
Size 25 mg
Catalog # 81406
US$ $154
Description

As maleimides, iodoacetamides are also among the most frequently used reagents for thiol modification. 5-IAF is one of the most popular fluorescent dyes for thiol modifications of proteins.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Baty JW, et al. (2002). Detection of oxidant sensitive thiol proteins by fluorescence labeling and two-dimensional electrophoresis. Proteomics 2, 1261-6; Moens PD, et al. (1994). Determination of the radial coordinate of Cys-374 in F-actin using fluorescence resonance energy transfer spectroscopy: effect of phalloidin on polymer assembly. Biochemitry 33, 13102-8.
Molecular Weight 515.25
Spectral Properties Abs/Em = 492/515 nm
Solvent System DMF or DMSO
Product Citations Schnell, F.J. et al. (2009). CD8+ T Cell Responses to a Viral Escape Mutant Epitope: Active Suppression via Altered SHP-1 Activity. J Immunol 182, 1829.
Wang, X. et al. (2009). Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice. J Cell Biol 184, 143.
     
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