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Assay Kits  >  MMP Assay Kits  >>  SensoLyte® 520 MMP-3 Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 MMP - 3 Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 71152
US$ $435
Description

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion and other diseases is well known. MMP-3 (stromelysin-1, transin-1) is proposed as a potential therapeutic target.

The SensoLyte® 520 MMP-3 Assay Kit uses a 5-FAM (fluorophore) and QXL®520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-3 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL®520. Upon the cleavage of the FRET peptide by MMP-3, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL®520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Kit size:100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Brochure
Storage -20°C
Product Citations Ozeki, N. et al. (2014). IL-1β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway. Exp Cell Res.
Yamaguchi, H. et al. (2014). Proinflammatory cytokines induce Stromelysin-1–mediated cell proliferation in dental pulp fibroblast-like cells. J Endodontics 40, 89. doi: http://dx.doi.org/10.1016/j.joen.2013.09.029.
Pifer, MA. et al. (2014). Matrix metalloproteinase content and activity in low-platelet, low-leukocyte and high-platelet, high-leukocyte platelet rich plasma (PRP) and the biologic response to PRP by human ligament fibroblasts. Am J Sports Med 42, 1122. doi: 10.1177/0363546514524710.
Shin, E. et al. (2012). Matrix metalloproteinase-3 is activated by HtrA2/Omi in dopaminergic cells: relevance to Parkinson's disease. Neurochem Int., 60, 249.
Dayoub, R. et al. (2011). Liver regeneration associated protein (ALR) exhibits antimetastatic potential in hepatocellular carcinoma. Mol Med 17, 221.
Koyama, Y. et al. (2008). Endothelins stimulate the production of stromelysin-1 in cultured rat astrocytes. Biochem Biophys Res Commun 371, 659.
Woo, M. et al. (2008). Inhibition of MMP-3 or -9 suppresses lipopolysaccharide-induced expression of proinflammatory cytokines and iNOS in microglia. J Neurochem 106, 770.
     
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