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Assay Kits  >  MMP Assay Kits  >>  SensoLyte® 520 MMP-8 Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 MMP - 8 Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 71154
US$ $435
Description

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-8 (neutrophil collagenase) is proposed as a potential anti-cancer drug target. SensoLyte® 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-8, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Kit size:100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Brochure
Storage -20°C
Product Citations Schubert-Unkmeir, A. et al. (2010). Neisseria meningitidis Induces Brain Microvascular Endothelial Cell Detachment from the Matrix and Cleavage of Occludin: A Role for MMP-8. PLoS Pathog 6, 4.
Slanina, H. et al. (2010). Real-time impedance analysis of host cell response to meningococcal infection. J Microbiol Met 84, 101.
     
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