TACE FRET Substrate I (DABCYL - Leu - Ala - Gln - Ala - Val - Arg - Ser - Ser - Ser - Arg - EDANS)
% Peak Area By HPLC ≥ 95%
TACE FRET substrate I is derived from the cleavage sequence of pro-TNFa recognized by TNFa-cleaving enzyme (TACE). TNFa is presumably shed from cell membranes by TNFa-cleaving enzyme (TACE). Incubation of the TACE substrate with recombinant human TACE gives a specific cleavage to restore the quenched fluorescence. The substrate is widely used to screen inhibitors of TNF-convertase.
Minimum Purity: 95%
Becker BF, et al. (2002). Application of peptides containing the cleavage sequence of pro-TNFalpha in assessing TACE activity of whole cells. Biol Chem 383, 1821-6, Mohler KM, et al. (1994). Protection against a lethal dose of endotoxin by an inhibitor of tumour necrosis factor processing. Nature 370, 218-20