Peptide sequence KKRFSFKKSFKL is derived from amino acid residues 154 –165 of myristoylated alanine-rich C-kinase substrate (MARCKS) protein phosphorylation site domain (PSD). It retains the first two of the four serine residues in the PSD. This peptide is proven to be a generic substrate for protein kinase C (including PKCα and β, naturally-occurring PKC δ, ε, and θ, as well as atypical PKC ξ) at Ser5 and Ser9, even though atypical PKCs do not utilize intact MARCKS as a substrate.
Ref: Irie, K. et al. Pharmacol. Ther. 93, 271 (2002); Uberall, F. et al. J. Biol. Chem. 272, 4072 (1997); Blackshear, P. J. Biol. Chem. 268, 1501 (1993).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
5 - TMR - Lys - Lys - Arg - Phe - Ser - Phe - Lys - Lys - Ser - Phe - Lys - Leu - OH