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Peptides  >  Fluorescent Labeled Peptides  >>  360 MMP FRET Substrate VI

Product Name 360 MMP FRET Substrate VI
Dnp - PLAYWAR
Size 1 mg
Catalog # 27106
US$ $71
Purity % Peak Area By HPLC ≥ 95%
Description

This substrate has a fluorescent Trp residue in subsite P2 and a dinitrophenol (Dnp) quenching group at the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-P1 and P3 is optimized for MMP-8, while Arg is placed in P4 to enhance solubility. Abs/Em = 280/360 nm.

Storage -20C
References Ref: Aschi, M. et al. J. Comput. Aided. Mol. Des. 16, 213 (2002); Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991).
Molecular Weight 1042.1
Sequence
(One-Letter Code)
Dnp-PLAYWAR
Sequence
(Three-Letter Code)
Dnp - Pro - Leu - Ala - Tyr - Trp - Ala - Arg - OH
     
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