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Fluorescent Dyes  >  Reactive Fluorescent Dyes  >>  LRB-SC

Product Name LRB - SC
[Lissamine™ rhodamine B sulfonyl chloride]
Size 1 g
Catalog # 81108-1000
US$ $193
Description

LissamineTM rhodamine B (LRB) sulfonyl chloride (SC) is a mixture of isomeric sulfonyl chlorides. Its fluorescence emission spectrum lies between those of tetramethylrhodamines and X-rhodamines, and it can be well excited by 568 nm line of the Ar-Kr mixed gas laser that is used in many confocal laser-scanning microscopes. Like FITC reagents, LRB-SC is relatively inexpensive. However, it is quite labile in basic solutions, making it somewhat difficult to achieve reproducible conjugations.

Storage -20°C desiccated and protected from light
References Smith SN and Steer RP (2001). The photophysics of Lissamine rhodamine–B sulfonyl chloride in aqueous solution: implications for fluorescent protein–dye conjugates. J Photochem Photobiol, A 139, 151; Neves C, et al. (2000). Novel method for covalent fluorescent labeling of plasmid DNA that maintains structural integrity of the plasmid. Bioconjug Chem 11, 51-5.
Molecular Weight 577.11
Spectral Properties Abs/Em = 568/584 nm
Solvent System DMF (do NOT use DMSO)
Product Citations Cabib, E. et al. (2008). Assembly of the Yeast Cell Wall. J Biol Chem 283, 29859.
Kosik, O. et al. (2008). One-pot fluorescent labeling of xyloglucan oligosaccharides with sulforhodamine. Anal Biochem 375, 232.
     
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