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Assay Kits  >  Epigenetics  >>  SensoLyte® 440 HDAC Activity Assay Kit *Fluorimetric*

Product Name SensoLyte® 440 HDAC Activity Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 72083
US$ $372
Description

Histone deacetylase (HDAC) enzymes modulate gene expression through the deacetylation of lysine residues on histone proteins and act as transcriptional repressors of genes. Based on their role in cell cycling, apoptosis and differentiation, HDACs have been chosen as therapeutic targets for the treatment of cancer and neurodegenerative diseases. The SensoLyte® 440 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a fluorogenic substrate Ac-GAK (Ac)-AMC. In the first step, an acetylated substrate is incubated with HDAC containing samples. Deacetylation of substrate sensitizes it, so that, in the second step mixing with the HDAC developer generates the AMC fluorophore, that can be detected at Ex/Em= 354 nm/442 nm. The substrate included in the kit is cell-permeable, and the assay can be used to measure HDAC activity directly in cell culture in a 96-well plate without a time-consuming cell extraction step. This kit also can be used for high throughput screening of HDAC inhibitors using extracts or purified enzymes. The kit contains: • Ac-GAK (Ac)-AMC HDAC substrate • Deacetylated reference standard for calibration • HeLa nuclear extract • Assay buffer • Cell lysis buffer • HDAC developer • Trichostatin A (HDAC inhibitor) • A detailed protocol 100 assays (96-well plate)

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C
     
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