Login
Existing Account

Please login first to complete purchase/ quotation request, view custom order reports, or create favorites list.

Customer ID:
Password:
Stay Logged In


Forgot your Customer ID or Password?
New Account

Don't have an account with us yet? Please set up an account to place order or obtain customer services.

Assay Kits  >  Epigenetics  >>  SensoLyte® 440 Deubiquitination Assay Kit *Fluorimetric*

Product Name SensoLyte® 440 Deubiquitination Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72204
US$ $495
Description

Deubiquitinating enzymes (DUBs) are proteases that reverse ubiquitin modifications and can rescue a protein from proteasomal degradation. They specifically cleave ubiquitin-linked molecules downstream of the terminal carbonyl of the last residue of ubiquitin (Gly76). DUBs are classified into five families. Well-defined families include ubiquitin carboxyl-terminal hydrolases (UCH) and ubiquitin-processing proteases (UBP). They act as regulators of diverse cellular processes, such as development, growth and DNA repair. DUBs have become actively studied molecular targets for drug discovery since they are implicated in the pathogenesis of many human diseases. The SensoLyte® 440 Deubiquitination Assay Kit provides a convenient assay for the screening of enzyme inhibitors and activators or for continuous assay of deubiquitination activity. A natural substrate, ubiquitin, coupled with the AMC (7-amino-4-methylcoumarin) fluorophore is used in the assay. Upon the cleavage by a deubiquitin protease, this substrate generates the blue fluorophore that can be detected at excitation/emission=354/442 nm. The kit contains: • The 440 DUB fluorogenic substrate (Ex/Em=354/442 nm upon cleavage) • Assay buffer • Deubiquitin protease UCH-L3 • Inhibitor • Fluorescence reference standard for calibration • A detailed protocol Kit size: 100 assays (96-well plate)

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
     
  < Back