This kit is optimized to detect human/mouse/rat alpha-Synuclein (α-Synuclein) in cell and tissue lysate, and in body fluids such as cerebrospinal fluid (CSF), plasma, and serum. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.
One Step Convenient Format
o Pre-coated and pre-blocked 96-well strip plate o Ready-to-use substrate solution and other assay components o One step assay (samples and detection antibody are added simultaneously) o 1 hour assay time at room temperature (excluding incubation time)
Minimal Sample Size
o Requires only 10-20 μl of cell and tissue lysate or body fluids
o Detects 5 pg/ml of α-Synuclein (calculated as three standard deviations from the blank)