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Assay Kits  >  Cathepsin Assay Kits  >>  SensoLyte® 520 Cathepsin L Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 Cathepsin L Assay Kit *Fluorimetric*  NEW
Size 1 kit
Catalog # 72218
US$ $435
Description

Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins. The SensoLyte® 520 Cathepsin L Activity Assay Kit uses a QXL® 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measuring enzyme activity. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. The QXL® 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of HiLyte™ Fluor 488 fluorescence and is monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, the optimal pH for Cathepsin L activity. Kit size: 100 assays (96-well plate)

Images SensoLyte<sup>®</sup> 520 Cathepsin L Assay Kit *Fluorimetric*
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C
     
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