Login
Existing Account

Please login first to complete purchase/ quotation request, view custom order reports, or create favorites list.

Customer ID:
Password:
Stay Logged In


Forgot your Customer ID or Password?
New Account

Don't have an account with us yet? Please set up an account to place order or obtain customer services.

Assay Kits  >  Alzheimer's and Parkinson's Disease  >>  SensoLyte® 520 BACE2 Activity Assay *Fluorimetric*

Product Name SensoLyte® 520 BACE2 Activity Assay *Fluorimetric*  NEW
Size 1 kit
Catalog # 72225
US$ $435
Description

BACE2 (β-Secretase-2, Memapsin-1) belongs to the family of transmembrane aspartic proteases. BACE2 has approximately 75% homology with BACE1 (β-Secretase-1) and is similar in that both cleaves Amyloid Precursor Protein (APP) at β-site generating N-terminal part of beta-Amyloid peptide. Unlike BACE1 that is highly expressed in pancreas and brain tissue, BACE2 is more widespread and can be found in many peripheral tissues at higher levels compared to BACE1. BACE2 is one of the highest efficiency beta-amyloid peptide degrading enzyme that is less active only to insulin degrading enzyme (IDE); therefore, targeting BACE2 is considered a potential therapeutic strategy for the prevention and treatment of Alzheimer's disease.

The SensoLyte® 520 BACE2 Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of BACE2 inhibitors. It contains a QXL® 520/ HiLyte™ Fluor488 FRET substrate, derived from a peptide sequence surrounding cleavage site of BACE2. QXL® 520 quenches HiLyte™ Fluor488 fluorescence in the intact FRET peptide. Active BACE2 cleaves FRET substrate into two separate fragments resulting in the release of HiLyte™ Fluor488 fluorescence that can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of HiLyte™ Fluor488 is less interfered by the autofluorescence of components in biological samples and test compounds. Kit Size: 100 assays (96-well plate)


RELATED PRODUCTS:
SensoLyte® 520 β-Secretase Activity Assay *Fluorimetric*
SensoLyte® 520 ADAM10 Activity Assay Kit *Fluorimetric*
SensoLyte® 520 Neprilysin Activity Assay Kit *Fluorimetric*
SensoLyte® Cathepsin Assays *Fluorimetric*
SensoLyte® Anti-α-Synuclein (Human, Mouse, Rat) ELISA Kit *Colorimetric*
SensoLyte® Anti-β-Amyloid (1-40) & (1-42), Human, Mouse/ Rat ELISA Kits *Colorimetric*
Recombinant human α & ß-Synucleins
β-Amyloid Peptides - Wide Selection

Product Spotlight: Tau Products - Antibodies, Recombinant Proteins and Peptides

Figure 1. Inhibition of BACE2 activity by β-secretase inhibitor IV as measured with SensoLyte® 520 BACE2 Assay Kit.

Images SensoLyte<sup>®</sup> 520 BACE2 Activity Assay <i>*Fluorimetric*</i>
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
     
  < Back