Fluorescent Dyes  >  Enzyme Detection Reagents
Proteolytic cleavage of peptide bonds is one of the most important mechanisms affecting the properties and functions of proteins. Proteases are pervasively distributed in all tissues and biological fluids. They play essential roles in protein activation, cell regulation and signaling. They also play a role in the generation of amino acids for protein synthesis or for use in other metabolic pathways.
AnaSpec offers one of the world's most extensive collections of chromogenic and fluorogenic substrates for you to analyze your target proteases and inhibitors. These include selective protease substrates for caspases, MMPs, secretases and viral proteases such as HIV and HCV. Our EnzoLyte™ protease assay kits provide speed, convenience and accuracy for analyzing protease activities and screening protease inhibitors. To further address your specific needs in protease research, we offer competitive custom synthesis and assay design for any proteases that might interest you.
 Product Size Catalog # US$  
(Z - AA)2Rh110
(Z - AA)2Rh110
5 mg 60320-5 $115
(Z - Ala - Ala - Ala - Ala)2Rh110
(Z - AAAA)2Rh110
5 mg 60321-5 $115
(Z - AR)2Rh110•2HCl
(Z - AR)2Rh110•HCl
5 mg 60322-5 $115
(Z - Arg)2Rh110 • 2HCl
(Z - R)2Rh110 • 2HCl
5 mg 60323-5 $115
2',7' - Dichlorodihydrofluorescein diacetate
H2DCFDA, H2DCF DA
100 mg 85706 $93
3 - Cyano - 7 - ethoxycoumarin 10 mg 85705 $66
360 MMP FRET Substrate I
Dnp - PLGLWAr - NH2
1 mg 27074 $99
360 MMP FRET Substrate I
Dnp - PLGLWAr - NH2
5 mg 27075 $341
360 MMP FRET Substrate II
[DNP - Pro - Leu - Ala - Leu - Trp - Ala - Arg - OH]
1 mg 27082 $71
360 MMP FRET Substrate II
Dnp - PLALWAR
5 mg 27083 $291
360 MMP FRET Substrate III
Dnp - PLGMWSR
1 mg 27094 $71
360 MMP FRET Substrate IV
Dnp - PYAYWMR
1 mg 27098 $71
360 MMP FRET Substrate IV
Dnp - PYAYWMR
5 mg 27099 $291
360 MMP FRET Substrate V
Dnp - RPLALWRS
1 mg 27102 $71
360 MMP FRET Substrate VI
Dnp - PLAYWAR
1 mg 27106 $71
360 MMP FRET Substrate VI
Dnp - PLAYWAR
5 mg 27107 $291
390 MMP FRET Substrate I
Mca - PLGL - Dpa - AR - NH2
1 mg 27076 $99
390 MMP FRET Substrate I
Mca - PLGL - Dpa - AR - NH2
5 mg 27077 $396
390 MMP FRET Substrate II
Mca - PLGL - Dpa - AR
5 mg 27079 $385
390 MMP FRET Substrate III
Mca - PLA - Nva - Dpa - AR - NH2
1 mg 27090 $99
390 MMP FRET Substrate IV
Mca - P - Cha - G - Nva - HA - Dpa - NH2
1 mg 27108 $99
390 MMP FRET Substrate IV
Mca - P - Cha - G - Nva - HA - Dpa - NH2
5 mg 27109 $385
4 - CN
[4 - Chloro - 1 - naphthol], 1M solution in DMSO
5 mL 85503 $66
4 - Methylumbelliferyl - β - D - glucoside 250 mg 85607 $61
4 - Nitrophenyl - α - D - galactopyranoside 1 g 85638 $66
4 - Nitrophenyl - N - acetyl - b - D - galactosaminide 100 mg 85618 $121
490 MMP FRET Substrate III
DABCYL - RPLALWRS - EDANS
1 mg 27104 $220
490 MMP FRET Substrate IV
DabcylPlus - KPLA - Nva - D(EDANS) - AR - NH2
0.1 mg 60637 $93
490 MMP FRET Substrate V
NBD - RPKPLA - Nva - WK(DMC) - NH2
1 mg 60563-1 $363
5 - (and - 6) - Carboxy - 2',7' - dichlorodihydrofluorescein diacetate 25 mg 85703 $93
5 - Carboxy - 2',7' - dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) 5 mg 85702 $93
5 - FAM MMP FRET Peptide Fluorescence Standard I
5 - FAM - PL
0.1 mg 60584-01 $115
520 MMP FRET Substrate I
QXL® 520 - PLGLWArK(5 - FAM) - NH2
0.1 mg 60568-01 $159
520 MMP FRET Substrate II
QXL® 520 - PLALWARK(5 - FAM) - NH2
0.1 mg 60569-01 $159
520 MMP FRET Substrate III
QXL® 520 - PLGC(Me)HAr - K(5 - FAM) - NH2
0.1 mg 60570-01 $159
520 MMP FRET Substrate IV
5 - FAM - PLA - Nva - Dap(QXL® 520) - AR - NH2
0.1 mg 60571-01 $159
520 MMP FRET Substrate IX
QXL® 520 - RPLALWRK(5 - FAM) - NH2
0.1 mg 60576-01 $159
520 MMP FRET Substrate V
5 - FAM - PLGL - Dap(QXL® 520) - AR - NH2
0.1 mg 60572-01 $159
520 MMP FRET Substrate VI
QXL® 520 - PLGMWSRK(5 - FAM) - NH2
0.1 mg 60573-01 $159
520 MMP FRET Substrate VII
QXL® 520 - PYAYWMRK(5 - FAM) - NH2
0.1 mg 60574-01 $159
520 MMP FRET Substrate VIII
QXL® 520 - RPKPLA - Nva - WK(5 - FAM) - NH2
0.1 mg 60575-01 $159
520 MMP FRET Substrate X
QXL® 520 - PLAYWARK(5 - FAM) - NH2
0.1 mg 60577-01 $159
520 MMP FRET Substrate XI
5 - FAM - P - Cha - G - Nva - HA - Dap(QXL® 520) - NH2
0.1 mg 60578-01 $159
520 MMP FRET Substrate XII
5 - FAM - RPKPYA - Nva - WM - K(QXL® 520) - NH2
0.1 mg 60579-01 $159
520 MMP FRET Substrate XIII
5 - FAM - RPKPVE - Nva - WRK(QXL® 520) - NH2
0.1 mg 60580-01 $159
520 MMP FRET Substrate XIV
QXL® 520 - γ - Abu - P - Cha - Abu - Smc - HA - Dab(5 - FAM) - AK - NH2 (Smc=S - Methyl - L - cysteine)
0.1 mg 60581-01 $159
520 MMP FRET Substrate XV
QXL® 520 - γ - Abu - PQGL - Dab(5 - FAM) - AK - NH2
0.1 mg 60582-01 $159
520 MMP FRET Substrate XVI
QXL® 520 - RPKPQQFWK(5 - FAM) - NH2
0.1 mg 60583-01 $159
520 MMP FRET Substrate XVII
QXL® 520 - KPLA - Nva - Dap(5 - FAM) - AR - NH2
0.1 mg 60554-01 $159
580 MMP FRET Substrate I
QXL® 570 - KPLA - Nva - Dap(5 - TAMRA) - AR - NH2
0.1 mg 60585-01 $159
7 - Ethoxy - 4 - trifluoromethylcoumarin 25 mg 85716 $66
Ac - IETD - AnaRed™ 1 mg 60314-1 $215
ADHP
10 - Acetyl - 3,7 - dihydroxyphenoxazine
25 mg 85500 $247
Alpha - Secretase Substrate 1
Mca - HQKLVFFAK(Dnp)
1 mg 60271 $253
Benzyloxyresorufin
[Resorufin benzyl ether]
10 mg 85726 $66
Beta - Secretase Substrate 1a
Mca - EVKVDAEF - K(Dnp)
1 mg 60268 $253
Beta - Secretase Substrate 2
Mca - EVKMDAEFK(Dnp)
1 mg 60269 $253
Casein, FITC Conjugated 5 mg 85100 $91
Casein, TAMRA Conjugated 5 mg 85101 $181
Caspase 1 (ICE) Substrate 1m, fluorogenic
Ac - YEVD - AMC
5 mg 25267-5 $154
Caspase 1 (ICE) Substrate 2, chromogenic
Ac - YVAD - pNA
5 mg 25259-5 $105
Caspase 1 (ICE) Substrate 2f, fluorogenic
Ac - YVAD - AFC
5 mg 25271-5 $181
Caspase 1 (ICE) Substrate 2f, fluorogenic
Ac - YVAD - AFC
25 mg 25271-25 $726
Caspase 1 (ICE) Substrate 2m, fluorogenic
Ac - YVAD - AMC
25 mg 25261-25 $605
Caspase 1 (ICE) Substrate 2m, fluorogenic
Ac - YVAD - AMC
5 mg 25261-5 $154
Caspase 1 (ICE) Substrate 2r, fluorogenic
(Ac - YVAD)2 - Rh110
1 mg 60310-1 $154
Caspase 1 (ICE) Substrate 3f, fluorogenic
Ac - WEHD - AFC
5 mg 25285-5 $181
Caspase 1 (ICE) Substrate 3f, fluorogenic
Ac - WEHD - AFC
25 mg 25285-25 $726
Caspase 1 (ICE) Substrate 3m, fluorogenic
Ac - WEHD - AMC
25 mg 25275-25 $605
Caspase 2 (ICH - 1) Substrate 1m, fluorogenic
Ac - VDVAD - AMC
5 mg 25255-5 $154
Caspase 3 (Apopain) Substrate 1, chromogenic
Ac - DEVD - pNA
5 mg 25263-5 $83
Caspase 3 (Apopain) Substrate 1f, fluorogenic
Ac - DEVD - AFC
5 mg 25273-5 $181
Caspase 3 (Apopain) Substrate 1f, fluorogenic
Ac - DEVD - AFC
25 mg 25273-25 $726
Caspase 3 (Apopain) Substrate 1m, fluorogenic
Ac - DEVD - AMC
25 mg 25262-25 $605
Caspase 3 (Apopain) Substrate 1m, fluorogenic
Ac - DEVD - AMC
5 mg 25262-5 $154
Caspase 3 (Apopain) Substrate 1r - z, fluorogenic
(Z - DEVD)2 - Rh110
5 mg 60304-5 $350
Caspase 6 (Mch2) Substrate 1f, fluorogenic
Ac - VEID - AFC
5 mg 25272-5 $181
Caspase 6 (Mch2) Substrate 1m, fluorogenic
Ac - VEID - AMC
5 mg 25256-5 $154
Caspase 6 (Mch2) Substrate 1r, fluorogenic
(Ac - VEID)2 - Rh110
1 mg 60308-1 $154
Caspase 8 Substrate 1, chromogenic
Ac - IETD - pNA
5 mg 25258-5 $105
Caspase 8 Substrate 1f, fluorogenic
Ac - IETD - AFC
5 mg 25270-5 $181
Caspase 8 Substrate 1f, fluorogenic
Ac - IETD - AFC
25 mg 25270-25 $726
Caspase 8 Substrate 1f - z, fluorogenic
Z - IETD - AFC
5 mg 60324-5 $154
Caspase 8 Substrate 1m, fluorogenic
Ac - IETD - AMC
5 mg 25257-5 $154
Caspase 8 Substrate 1m, fluorogenic
Ac - IETD - AMC
25 mg 25257-25 $605
Caspase 8 Substrate 1r - z, fluorogenic
(Z - IEHD)2 - Rh110
5 mg 60312-5 $237
Caspase 8 Substrate 1r - z, fluorogenic
(Z - IETD)2 - Rh110
1 mg 60312-1 $154
Caspase 9 Substrate 1, chromogenic
Ac - LEHD - pNA
5 mg 25278-5 $105
Caspase 9 Substrate 1f, fluorogenic
Ac - LEHD - AFC
5 mg 25276-5 $181
Caspase 9 Substrate 1f, fluorogenic
Ac - LEHD - AFC
25 mg 25276-25 $726
Caspase 9 Substrate 1r, fluorogenic
(Ac - LEHD)2 - Rh110
5 mg 60306-5 $215
Caspase 9 Substrate 1r, fluorogenic
(Ac - LEHD)2 - Rh110
1 mg 60306-1 $154
Caspase 9 Substrate 2m, fluorogenic
Ac - LEHD - AMC
5 mg 25286-5 $181
Caspase 9 Substrate 2m, fluorogenic
Ac - LEHD - AMC
25 mg 25286-25 $726
Collagen (Type I), FITC conjugated *Water Insoluble* 5 mg 85102 $91
Collagen (Type I), FITC conjugated *Water Soluble* 1 mg 85111 $247
Collagen (Type IV), FAM conjugated 1 mg 85112 $323
D - Luciferin, free acid *UltraPure Grade* 25 mg 82250 $83
D - Luciferin, potassium salt *UltraPure Grade* 25 mg 82252 $105
D - Luciferin, sodium salt 25 mg 82251 $93
Dihydroethidium
[Hydroethidine], 5 mM solution in DMSO
1 mL 85718 $154
Dihydroethidium
[Hydroethidine] *Special Air - free Packaging*
10 mg 85717 $193
Dihydrofluorescein diacetate
[H2FDA]
25 mg 85710 $66
Dihydrorhodamine 123 10 mg 85711 $93
Dihydrorhodamine 123 *Air - free Packaging* 10 mg 85720 $159
Dihydrorhodamine 123, 5 mM solution in DMSO 1 mL 85719 $154
Dihydrorhodamine 6G 25 mg 85712 $93
Elastin, FITC Conjugated 1 mg 85113 $121
Elastin, FITC - Conjugated *Water Insoluble* 1 mg 85103 $181
Ethoxyresorufin
[Resorufin ethyl ether]
5 mg 85715 $66
FDG
[Fluorescein di - β - D - galactopyranoside]
5 mg 85600 $121
Gelatin, FITC Conjugated 5 mg 85145 $91
HCV Protease FRET Substrate (RET S1)
Ac - DE - D(Edans) - EE - Abu - ψ - [COO] - AS - K(Dabcyl) - NH2
0.25 mg 22991-025 $181
HCV Protease FRET Substrate (RET S1) 1 mg 22991 $583
HIV Protease FRET Substrate I 1 mg 22992 $231
HPPA
[3 - (4 - Hydroxyphenyl)propionic acid]*UltraPure Grade*
1 g 85505 $93
Malaria Aspartyl Proteinase FRET Substrate I
[DABCYL - Glu - Arg - Nle - Phe - Leu - Ser - Phe - Pro - EDANS]
1 mg 24480 $220
Malaria Aspartyl Proteinase FRET Substrate I
[DABCYL - Glu - Arg - Nle - Phe - Leu - Ser - Phe - Pro - EDANS]
5 mg 24481 $869
Mca MMP FRET Peptide Fluorescence Standard I
Mca - PL
1 mg 27159 $39
Mca MMP FRET Peptide Fluorescence Standard II
Mca - RPKPQ
1 mg 27112 $61
Mca MMP FRET Peptide Fluorescence Standard II
Mca - RPKPQ
5 mg 27113 $242
Methoxyresorufin
[Resorufin methyl ether]
5 mg 85707 $66
MMP Colorimetric Substrate I
Ac - PLG - SCH[CH2CH(CH3)2] - CO - LG - OC2H5
1 mg 27096 $121
MMP Colorimetric Substrate I
Ac - PLG - SCH[CH2CH(CH3)2] - CO - LG - OC2H5
5 mg 27097 $484
MMP Peptide Inhibitor I
Ac - RCGVPD - NH2
1 mg 27157 $49
MMP Peptide Inhibitor I
Ac - RCGVPD - NH2
5 mg 27158 $193
MPTS
[8 - Methoxypyrene - 1,3,6 - trisulfonic acid, trisodium salt]
100 mg 85725 $66
MUGlcU
[4 - Methylumbelliferyl - β - D - glucuronide]
100 mg 85606 $66
MUP
[4 - Methylumbelliferyl phosphate, free acid] *UltraPure Grade*
1 g 85310 $93
MUP, DCA
[4 - Methylumbelliferyl phosphate, dicyclohexylammonium salt, trihydrate] *UltraPure Grade*
1 g 85311 $93
NFF - 2
Mca - RPKPYA - Nva - WM - K(Dnp) - NH2
5 mg 27111 $385
NFF - 3
Mca - RPKPVE - Nva - WR - K(Dnp) - NH2
1 mg 27114 $99
NFF - 3
Mca - RPKPVE - Nva - WR - K(Dnp) - NH2
5 mg 27115 $385
Pentoxyresorufin
[Resorufin pentyl ether]
5 mg 85728 $66
pNPP
[4 - Nitrophenyl phosphate, disodium salt] *UltraPure Grade*
1 g 85301 $121
Renin FRET Substrate I
(DABCYL - g - Abu - Ile - His - Pro - Phe - His - Leu - Val - Ile - His - Thr - EDANS)
1 mg 24478 $220
Resazurin, sodium salt *UltraPure Grade* 100 mg 85740 $66
Resorufin β - D - Galactopyranoside 25 mg 85617 $93
TACE FRET Substrate I
(DABCYL - Leu - Ala - Gln - Ala - Val - Arg - Ser - Ser - Ser - Arg - EDANS)
5 mg 25349 $946
TACE FRET Substrate I
(DABCYL - Leu - Ala - Gln - Ala - Val - Arg - Ser - Ser - Ser - Arg - EDANS)
1 mg 25043 $237
TNO113
DABCYL - (γ - Abu) - P - Cha - Abu - Smc - HA - E(EDANS) - AK - NH2 (Smc=S - Methyl - L - Cysteine)
1 mg 27061 $291
TNO211
DABCYL - (γ - Abu) - PQGL - E(EDANS)AK - NH2
1 mg 25350 $253
TNO211
DABCYL - (γ - Abu) - PQGL - E(EDANS)AK - NH2
5 mg 25350-5 $1017
WAAG - 3R, Aggrecanase (ADAM - TS - 4) FRET Substrate
Abz - TEGEARGSVI - Dpa - KK - NH2
1 mg 60431-1 $237
X - Gal
[5 - Bromo - 4 - chloro - 3 - indolyl - β - D - galactopyranoside]
1 g 85620 $180
X - GlcU
[5 - Bromo - 4 - chloro - 3 - indoxyl - β - D - glucuronic acid, sodium salt]
25 mg 85630 $121
X - GluNAc
[5 - Bromo - 4 - chloro - 3 - indoxyl - N - acetyl - β - D - glucosaminide]
25 mg 85623 $121
Z - DEVD - AFC 5 mg 21685-5 $181
Z - DEVD - AFC 25 mg 21685-25 $726
Z - DEVD - AMC
Z - DEVD - AMC
5 mg 60303-5 $181
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