AnaSpec News

2 New Assay Kits - January 4, 2013

AnaSpec is pleased to announce the release of two (2) new SensoLyte® assay kits for the measurement of Cathepsin L activity.

Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins.

SensoLyte® 520 Cathepsin L Assay Kit *Fluorimetric* - Cat# 72218
The SensoLyte® 520 Cathepsin L Activity Assay Kit uses a QXL™ 520/HiLyte Fluor™ 488 labeled FRET peptide substrate for measuring enzyme activity. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. The QXL™ 520/HiLyte Fluor™ 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of HiLyte Fluor™ 488 fluorescence and is monitored at excitation/emission = 490/520 nm. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, the optimal pH for Cathepsin L activity.
Kit size: 100 assays

SensoLyte® Rh110 Cathepsin L Assay Kit *Fluorimetric* - Cat# 72217
The SensoLyte® Rh110 Cathepsin L Activity Assay Kit provides a convenient assay for screening Cathepsin L inhibitors or assay of enzyme activity using a fluorogenic substrate. Upon cleavage by Cathepsin L, this substrate generates the Rh110 (rhodamine 110) fluorophore with a bright green fluorescence that can be detected at excitation/emission=490/520 nm. The longer wavelength spectra and higher extinction coefficient of Rh110 provide greater sensitivity and less interference from other reaction components.
Kit size: 100 assays

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