AnaSpec News

Calcein AM for Live Cell Staining

t-PA acts as a cytokine to regulate lymphocyte–endothelium adhesion in experimental autoimmune encephalomyelitis is one of at least seven publications in 2014 citing the use of Calcein AM to date (June 27, 2014). This paper appeared in Clin Immunol 152, 90. doi: 10.1016/j.clim.2014.03.004 and is authored by Jinghua Wang, et al.

Calcein AM (Calcein acetoxymethyl ester), a non-fluorescent, cell permeable compound, converts to the strongly green fluorescent calcein when hydrolyzed by intracellular esterases in live cells. It is widely used for determining cell viability since the fluorescence intensity of calcein is proportional to the amount of live cells.

AnaSpec provides Calcein AM at two purity grades, >90% or >95%, as determined by HPLC.

• Calcein AM in ultra-pure grade, >95% or at >90% purity
• Premium quality manufactured under stringent QC standards
• Manufactured in California, USA
• Special packaging available
• Bulk quantities available
AnaSpec also offers:
SensoLyte® Calcein Cell Viability Assay Kit - a rapid and sensitive assay to detect viable cells. This kit provides ample reagents (dye and assay buffer) for 1000 assays in a 96-well plate format.

SensoLyte® AMC tPA Activity Assay Kit - a fluorimetric assay for detecting tPA enzyme activities (Ex/Em=354/442 nm). This kit provides ample reagents to perform 100 assays in a 96-well format.
2014 Citations of Calcein AM to date:
Boyer-Di Ponio, J. et al. (2014). Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PloS One 9, e84179

Billiet, T. et al. (2014). The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability. Biomat 35, 49. doi: 10.1016/j.biomaterials.2013.09.078.

Douglas, TE. et al. (2014). Generation of composites for bone tissue‐engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means. J Tissue Eng Regen Med. doi: 10.1002/term.1875

Gevaert, E. et al. (2014). Galactose‐functionalized gelatin hydrogels improve the functionality of encapsulated Hepg2 cells. Macromol Biosci 14, 419. doi: 10.1002/mabi.201300320

Samal, SK. et al. (2014). Enzymatic mineralization of silk scaffolds. Macromol Biosci. Doi: 10.1002/mabi.201300513

Sheats, MK. et al. (2014). Myristoylated Alanine Rich C Kinase Substrate (MARCKS) is essential to β2-integrin dependent responses of equine neutrophils. Vet Immunol Immunopathol

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