AnaSpec News

BD™ Fluorescent Dyes Manufactured by AnaSpec - September 17, 2008

AnaSpec announced today that BD Biosciences, a business segment of BD (Becton Dickinson and Company), has chosen it to manufacture and supply the BD™ H7, Calcein AM and DiIC12(3) Fluorescent Dyes.

“BD Biosciences has a tremendous reputation in the industry for innovative products of the highest quality,” stated Dr. Anita Hong, AnaSpec’s president. “We are proud to leverage AnaSpec’s fluorescent dye technology in the production of these novel dyes.”

H7 is the abbreviation of HiLyte FluorTM 750 Bis-NHS ester, which carries two amine-reactive groups, succinimidyl esters, on each dye molecule for general labeling of protein amine groups.

BD APC-H7 is a tandem conjugate and an analog of APC-CyTM7 with the same spectral properties. It has decreased intensity but it is engineered for greater stability and less spillover in the APC channel and consequently offers better performance than APC-Cy7. It has an absorption maximum of approximately 650 nm. When excited by light from a red laser, the APC fluorochrome can transfer energy to the cyanine dye, which then emits at a longer wavelength. The resulting fluorescent emission maximum is approximately 767 nm.

The cell viability indicator calcein AM, is a non-fluorescent, cell permeant compound that is hydrolyzed by intracellular esterases into the fluorescent anion calcein. BD™ Calcein AM Fluorescent Dyes can be used to fluorescently pre- and post-label viable cells to perform kinetic and endpoint experiments, respectively. DiIC12(3) is a lipophilic neuronal tracer, which is commonly used for the labeling of neuronal projections as well as lipid bilayers in other cell types. Since DiIC12(3) exhibits low toxicity and minimal effects on cell viability, this dye can be used to pre-label cells for a variety of applications.

BD™ DiIC12(3) Fluorescent Dye can be used to fluorescently label viable cells for assays, such as tumor cell invasion or endothelial cell migration. Moreover, cells are known to tolerate different fluorescent dyes for varying amounts of time in culture. While cells labeled with calcein AM should be exposed to the dye for less than eight hours, DiIC12(3) can be used for several days in culture without adversely affecting cells.

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