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Assay Kits  >  MMP Assay Kits  >>  SensoLyte ® 520 MMP-2 Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 MMP - 2 Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-71151
US$ $457

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion is well known. MMP-2 (gelatinase A) is proposed as a potential therapeutic target. SensoLyte® 520 MMP-2 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrate for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-2, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Kit size:100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C
Product Citations Liu, B. et al. (2014). Endothelin A receptor antagonism enhances inhibitory effects of anti-ganglioside GD2 monoclonal antibody on invasiveness and viability of human osteosarcoma cells. PloS one 9, e93576.

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Jo, YK. et al. (2011). ARP101, a selective MMP-2 inhibitor, induces autophagy-associated cell death in cancer cells. Biochem Biophys Res Commun 404, 1039.

Qazi, H. et al. (2011). Fluid shear stress regulates the invasive potential of glioma cells via modulation of migatory activity and matrix metalloproteinase expression. PLoS One 6, e20348.

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Rahn, D. et al. (2009). Failure of pelvic organ support in mice deficient in fibulin-3. Am J Pathol 174, 206.

Shi, Z-D. et al. (2009). Interstitial flow promotes vascular fibroblast, myofibroblast, and smooth muscle cell motility in 3-D collagen I via upregulation of MMP-1. Am J Physiol Heart Circ Physiol 10.1152/ajpheart.00369.2009.

Wieslander, C. et al. (2009). Quantification of pelvic organ prolapse in mice: Vaginal protease activity precedes increased MOPQ scores in fibulin 5 knockout mice. Biol Reprod 80, 407.

Johnson, SL. et al. (2008). A high-throughput screening approach to anthrax lethal factor inhibition. Chem Bio Drug Design 71, 131.

Wieslander, S. et al. (2008). Regulation of elastolytic proteases in the mouse vagina during pregnancy, parturition, and puerperium. Biol Reprod 78, 521.
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