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Assay Kits  >  MMP Assay Kits  >>  SensoLyte ® 520 Generic MMP Activity Kit *Fluorimetric*

Product Name SensoLyte ® 520 Generic MMP Activity Kit *Fluorimetric*
Size 1 kit
Catalog # AS-71158
US$ $457

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known.

The SensoLyte® 520 Generic MMP Assay Kit is optimized to detect the activity of a variety of MMPs, including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14. Compared to the SensoLyte® 520 kits designed for individual MMP, the Generic MMP Assay kit is 5 to 20 fold more sensitive, although less specific. This kit is ideal for detecting generic MMP activity in biological samples or for high throughput screening of MMPs’ inducers and inhibitors using purified MMPs. This kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by SensoLyte®. Upon the cleavage of the FRET peptide by MMPs, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Kit size:100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C
Product Citations Yang, Y. F.,et al. (2016). Effects of induction and inhibition of matrix cross-linking on remodeling of the aqueous outflow resistance by ocular trabecular meshwork cells. Scientific Reports, 6. doi: 10.1038/srep30505

Keller, KE. et al. (2014). Interleukin-20 receptor expression in the trabecular meshwork and its implication in glaucoma. J Ocul Pharmacol Th 30, 267.

Poormasjedi-Meibod, MS. et al. (2014). Anti-scarring properties of different tryptophan derivatives. PloS one 9, e91955.

Vidal, CM. et al. (2014). Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins. Acta Biomater 10, 3288.

Ma, B. et al. (2013). T Cell Factor 4 is a pro-catabolic and apoptotic factor in human articular chondrocytes by potentiating nuclear factor {kappa}B signaling. J Biol Chem 288, 17552. doi: 10.1074/jbc.M113.453985 .

Margarinos, NJ. et al. (2013). Mast cell–restricted, tetramer-forming tryptases induce aggrecanolysis in articular cartilage by activating matrix metalloproteinase-3 and -13 zymogens. J Immunol 191, 1404. doi: 10.4049/​jimmunol.1300856.

Frankwich, K. et al. (2012). Proof of Concept: Matrix metalloproteinase inhibitor decreases inflammation and improves muscle insulin sensitivity in people with type 2 diabetes. J Inflamm 9, 35. doi:10.1186/1476-9255-9-35

Li, R. et al. (2012). A dynamic model of calcific nodule destabilization in response to monocyte- and oxidized lipid-induced matrix metalloproteinases. Am J Physiol Cell Physiol 302:C658

Ma, M. et al. (2012). A Wnt/ß-catenin negative feedback loop inhibits IL-1-induced MMP expression in human articular chondrocytes. Arthritis Rheumatism 64, 2589.

Mayrand, D. et al. (2012). Angiogenic properties of myofibroblasts isolated from normal human skin wounds. Angiogenesis. Feb 18. [Epub ahead of print]

Oyama, JI. et al. (2012). Repetitive hyperthermia attenuates progression of left ventricular hypertrophy and increases telomerase activity in hypertensive rats. Am J Physiol Heart Circ Physiol. Mar 16. [Epub ahead of print]

Spolidoro, M. et al. (2012). Inhibition of matrix metalloproteinases prevents the potentiation of nondeprived-eye responses after monocular deprivation in juvenile rats. Cereb Cortex 22, 725. doi:10.1093/cercor/bhr158

Chabaud, S. et al. (2011). Decreased secretion of MMP by non-lesional late-stage scleroderma fibroblasts after selection via activation of the apoptotic fas-pathway. J Cell Physiol 226, 1907.

Hammoud, L. et al. (2011). Deficiency in TIMP-3 incresases cardiac rupture and mortality post-myocardial infarction via EGFR signaling beneficial effects of cetuximab. Basic Res Cardiol 106, 459.

Saleh, MA. et al. (2011). Distinct actions of ETA selective versus combined ETA/ETB receptor antagonists in early diabetic kidney disease. J Pharmacol 10.1124/jpet.111.178988.

Motz, G. et al. (2010). Chronic cigarette smoke exposure generates pathogenic T cells capable of driving COPD-like disease in Rag2-/- mice. Am J Respir Critical Care Med 181, 1233.

Razavian, M. et al. (2010). Molecular imaging of matrix metalloproteinase activation to predict murine aneurysm expansion in vivo. J Nucl Med 51, 1107.

Corriveau, MP. et al. (2009). The fibrotic phenotype of systemic sclerosis fibroblasts varies with disease duration and severity of skin involvement: reconstitution of skin fibrosis development using a tissue engineering approach. J Pathol 217, 534.

Hammoud, L. et al. (2009). Tissue inhibitor of metalloproteinase-3 inhibits neonatal mouse cardiomyocyte proliferation via EGFR/JNK/SP-1 signaling. Am J Physiol Cell Physiol 296, C735.

Santisteban, M. et al. (2009). Immune-induced epithelial to mesenchymal transition in vivo generates breast cancer stem cells. Cancer Res 69, 2887.

Errami, M. et al. (2008). Doxycycline attenuates isoproterenol- and transverse aortic banding-induced cardiac hypertrophy in mice. J Pharmacol Exp Ther 324, 1196

Adiseshaiah, P. et al. (2008). A Fra-1 dependent, matrix metalloproteinase driven EGFR activation promotes human lung epithelial cell motility and invasion. J Cell Physiol 216, 405.

Gueders, MM. et al. (2008). A novel formulation of inhaled doxycycline reduces allergen-induced inflammation, hyperresponsiveness and remodeling by matrix metalloproteinases and cytokines modulation in a mouse model of asthma. Biochem Pharmacol 75, 514.

Thal, DR. et al. (2008). Expression of coronin-3 (coronin-1C) in diffuse gliomas is related to malignancy. J Pathol 214, 415.
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