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Fluorescent Dyes  >  Fluorescent Physiological and Neurological Indicators  >>  HPTS

Product Name HPTS
[8 - Hydroxypyrene - 1,3,6 - trisulfonic acid, trisodium salt]
Size 1 g
Catalog # AS-84610
US$ $93

Cell-impermeable fluorescent pH indicator for slightly acidic pH range, pKa = 6.4

Detailed Information Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Biswas S, et al. (2004). Quenching of fluorescence of 1-hydroxypyrene-3,6,8-trisulfonate (HPTS) by Cu2+, Co2+, Ni2+, I-, and cetylpyridinium (CP+) ions in water/AOT/heptane microemulsion. J Colloid Interface Sci 271, 157-62, Ma LY, et al. (2004). A long lifetime chemical sensor: study on fluorescence property of fluorescein isothiocyanate and preparation of pH chemical sensor. Spectrochim Acta A Mol Biomol Spectrosc 60, 1865-72, Kermis HR, et al. (2003). Rapid method for the preparation of a robust optical pH sensor. Analyst 128, 1181-6, Kermis HR, et al. (2002). Dual excitation ratiometric fluorescent pH sensor for noninvasive bioprocess monitoring: development and application. Biotechnol Prog 18, 1047-53, Ozkan P and Mutharasan R (2002). A rapid method for measuring intracellular pH using BCECF-AM. Biochim Biophys Acta 1572, 143-8, Marechal X, et al. (1999). In vivo application of intestinal pH measurement using 2'',7''-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence imaging. Photochem Photobiol 70, 813-9, Weinlich M, et al. (1998). Simultaneous detection of cell volume and intracellular pH in isolated rat duodenal cells by confocal microscopy and BCECF. Res Exp Med (Berl) 198, 73-82, Boyarsky G, et al. (1996). Inadequacy of high K+/nigericin for calibrating BCECF. I. Estimating steady-state intracellular pH. Am J Physiol 271, C1131-45, Boyarsky G, et al. (1996). Inadequacy of high K+/nigericin for calibrating BCECF. II. Intracellular pH dependence of the correction. Am J Physiol 271, C1146-56, Carrero J and Voss EW, Jr. (1996). Temperature and pH dependence of fluorescein binding within the monoclonal antibody 9-40 active site as monitored by hydrostatic pressure. J Biol Chem 271, 5332-7.
Molecular Weight 524.4
Spectral Properties Abs/Em = 454/511 nm
Solvent System water
Product Citations Kawano, S. et al. (2010). Construction of Continuous Porous Organogels, Hydrogels, and Bicontinuous Organo/Hydro Hybrid Gels from Bicontinuous Microemulsions. Macromolecules 43, 473.
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