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Fluorescent Dyes  >  Fluorescent Physiological and Neurological Indicators  >>  Calcein, AM

Product Name Calcein, AM
CAS 148504-34-1
Size 1 mg
Catalog # AS-89201
US$ $139
Purity ≥95% by HPLC

Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells. Hela cells stained with AnaSpec’s Calcein AM fluorescent dye (Cat# AS-89201, AS-89202, AS-89203 and AS-89204).

MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# AS-89202). Images acquired every 3 seconds and total elapsed time on video is 12 min.

Images Calcein, AM
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C desiccated and protected from light
Molecular Weight 994.857
Molecular Formula C46H46N2O23
Spectral Properties Abs/Em = 494/517 nm
Solvent System DMSO
Product Citations Graham, DM. et al. (2012). Spatial manipulation of cells and organelles using single electrode dielectrophoresis. Int J of Life Sci Meth 52 , 39-43

Kim, H. et al. (2011). Human macrophage ATP7A is localized in the trans-golgi apparatus, controls intracellular copper levels, and mediates macrophage responses to dermal wounds. Inflammation 10.1007/s10753-011-9302-z.

Decaris, ML. et al. (2010). Design of experiments approach to engineer cell-secreted matrices for directing osteogenic differentiation. Annals Biomed Eng 39, 1174.

Hernlem, B. et al. (2010). Dual fluorochrome flow cytometric assessment of yeast viability. Current Microbiol 61, 57.

Miura, Y. et al. (2010). Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture. Opthalmic Res 43, 122.

Zaveri, T. et al. (2010). Contributions of surface topography and cytotoxicity to the macrophage response to zinc oxide nanorods. Biomater 31, 2999.

Decaris, ML. et al. (2009). Influence of the oxygen microenvironment on the proangiogenic potential of human endothelial colony forming cells. Angiogenesis 12, 303.

Delaunay, JL. et al. (2009). A missense mutation in ABCB4 gene involved in progressive familial intrahepatic cholestasis type 3 leads to a folding defect that can be rescued by low temperature. Hepatology 49, 1218.

Zhang, K-H. et al. (2009). Ferritin heavy chain–mediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition. Cancer Res. 10.1158/0008-5472.CAN-09-0112.
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