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Assay Kits  >  MMP Assay Kits  >>  SensoLyte ® Plus 520 MMP-1 Assay Kit *Fluorimetric and Enhanced Selectivity*
Product Name SensoLyte ® Plus 520 MMP - 1 Assay Kit *Fluorimetric and Enhanced Selectivity*
Size 1 kit
Catalog # AS-72012
US$ $589
Description

The SensoLyte® Plus 520 MMP-1 Assay Kit is designed for specifically detecting MMP-1 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-1 monoclonal antibody is used in combination with an MMP fluorogenic substrate, 5-FAM/QXL®520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-1-induced cleavage of the FRET substrate. Ample materials are provided to perform 96 assays in a 96-well format.
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Poster
References Jian J, Pelle E, Yang Q, Pernodet N, Maes D, Huang X. Iron sensitizes keratinocytes and fibroblasts to UVA-mediated matrix metalloproteinase-1 through TNF-? and ERK activation. Exp Dermatol. 2011 Mar; 20(3):249-54. PMID:20701626
Product Citations Carrizzo, A. et al. (2015). Pentraxin 3 induces vascular endothelial dysfunction through a P-selectin/Matrix Metalloproteinase-1 pathway. Circulation 131, 1495. doi: 10.1161/CIRCULATIONAHA.114.014822.

Atay, S. et al. (2014). Oncogenic KIT-containing exosomes increase gastrointestinal stromal tumor cell invasion. PNAS 111, 711. doi: 10.1073/pnas.1310501111.

Halvorsen, B. et al. (2014). Increased levels of CCR7 ligands in carotid atherosclerosis: different effects in macrophages and smooth muscle cells. Cardiovasc Res 102, 148. doi: 10.1093/cvr/cvu036.

Mogami, H. et al. (2014). Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth. J Biol Chem 289, 13295. doi: 10.1074/jbc.M114.550541.

Ozeki, N. et al. (2014). IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells. Exp Cell Res 323, 165.

Mogami, H. et al. (2013) Fetal fibronectin signaling induces Matrix Metalloproteases and Cyclooxygenase-2 (COX-2) in amnion cells and preterm birth in mice JBC 2883,1953 doi:10.1074/jbc.M112.424366

Bogatkevich, GS. et al. (2012). The PPARγ agonist Rosiglitazone is antifibrotic for scleroderma lung fibroblasts: mechanisms of action and differential racial effects. Pulmonary Med doi:10.1155/2012/545172

Leong, D. et al. (2011). Physiological loading of joints prevents cartilage degradation through CITED2. FASEB J 25, 182.

Park, C. et al. (2011). Epidermal growth factor-induced matrix metalloproteinase-1 expression is negatively regulated by p38 MAPK in human skin fibroblasts. J Dermatol Sc 64, 134.

Handy, J. et al. (2010). Adiponectin activation of AMPK disrupts leptin-mediated hepatic fibrosis via suppressors of cytokine signaling (SOCS-3). J Cell Biochem 110, 1195.

Cowan, RW. et al. (2009). Collagenase expression and activity in the stromal cells from giant cell tumour of bone. Bone 44, 865.

Jian, J. et al. (2011). Iron sensitizes keratinocytes and fibroblasts to UVA-mediated matrix metalloproteinase-1 through TNF-α and ERK activation. Exp Dermatol 20, 249.

Moor, A. et al. (2009). Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers. Wound Repair Regen 17, 832.

Shi, Z-D. et al. (2009). Interstitial flow promotes vascular fibroblast, myofibroblast, and smooth muscle cell motility in 3-D collagen I via upregulation of MMP-1. Am J Physiol Heart Circ Physiol 10.1152/ajpheart.00369.2009.

Qazi, H. et al. (2011). Fluid shear stress regulates the invasive potential of glioma cells via modulation of migatory activity and matrix metalloproteinase expression. PLoS One 6, e20348.
     
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