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Assay Kits  >  Viral/Bacterial Protease Assay Kits - HIV, HCV, TEV, WNV, Sortase, Furin  >>  SensoLyte ® 520 HIV Protease Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 HIV Protease Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-71147
US$ $457

The SensoLyte® 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte™ Fluor 488 (fluorophore) and QXL® 520 (quencher) for continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of HiLyte™ Fluor 488 is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this HiLyte™ Fluor 488/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components, thus providing better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: • HiLyte™ Fluor 488/QXL® 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) • Assay buffer • HIV protease inhibitor • Fluorescence reference standard for calibration • A detailed protocol

Kit Size: 100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Meng, J. et al. (2015). Screening of HIV-1 protease using a combination of an ultra-high-throughput fluorescent-based assay and RapidFire mass spectrometry. J Biomol Screen 20, 606. doi: 10.1177/1087057115570838.

Lu, D. et al. (2010). Design, asymmetric synthesis, and evaluation of pseudosymmetric sulfoximine inhibitors against HIV-1 protease. Bioorg Med Chem 18, 2037. doi: 10.1016/j.bmc.2010.01.020.

Wei, Y. et al. (2009). Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases. Bioorganic and Medicinal Chem 17, 3003.
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