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Assay Kits  >  Viral/Bacterial Protease Assay Kits - HIV, HCV, TEV, WNV, Sortase, Furin  >>  SensoLyte ® 520 HCV Protease Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 HCV Protease Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-71145
US$ $457

The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL® 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: • 5-FAM/QXL® 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) • Fluorescence reference standard for calibration • A detailed protocol

Kit Size: 100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Pessoa, L. et al. (2016) Development of a rapid phenotypic test for HCV protease inhibitors with potential use in clinical decisions. Genetics & Molecular Biology, 39, 3, 358-364

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Salim, MTA. et al. (2011). Potent and selective inhibition of hepatitis C virus replication by novel phenanthridinone derivatives. Biochem Biophys Res Commun 415, 714.

Ma, C. et al. (2009). HCV Protease Inhibitory, Cytotoxic and Apoptosis-Inducing Effects of Oleanolic Acid Derivatives. J Pharm Pharmaceut Sci 12, 243.

Phuong, DT. et al. (2009). Inhibitory effects of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. Phytother. Res. 23, 582.

Wang, S-Y. et al. (2009). Bioactivity-guided screening identifies pheophytin a as a potent anti-hepatitis C virus compound from Lonicera hypoglauca Miq. BBRC 385, 230.

Wei Y, et al. (2009). Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases. Bioorg Med Chem. 17, 3003.

Ma, Y. et al. (2008). NS3 helicase domains involved in infectious intracellular Hepatitis C Virus particle assembly. J. Virol. 82, 7624.

Ma, C. et al. (2007). Triterpenes from Cynomorium songaricium — analysis of HCV protease inhibitory activity, quantification, and content change under the influence of heating. J Nat Med 63, 9.
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