SensoLyte ® MFP Protein Phosphatase Assay Kit *Fluorimetric*
Protein phosphatases have received great attention as drug-screening targets. MFP is a proprietary fluorogenic substrate for most phosphatases, i.e., alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases, and serine/threonine phosphatases. The phosphatase-induced hydrolysis of MFP yields an intense fluorescent product that has excitation wavelength around 488 nm, and maximum emission around 520 nm. This characteristic makes the substrate adaptable to all the fluorescence instruments. SensoLyte® MFP Protein Phosphatase Assay Kit uses MFP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. The kit contains: • MFP phosphatase substrate • Protein phosphatase inhibitor • Assay buffer • A 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.