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Assay Kits  >  Viral/Bacterial Protease Assay Kits - HIV, HCV, TEV, WNV, Sortase, Furin  >>  SensoLyte ® 490 HCV Protease Assay Kit *Fluorimetric*

Product Name SensoLyte ® 490 HCV Protease Assay Kit *Fluorimetric*
Kit Size: 200 assays
Size 1 kit
Catalog # AS-72087
US$ $457

HCV protease is identified as an important drug-screening target. The SensoLyte® 490 HCV Protease Assay Kit uses an optimized FRET peptide substrate for the continuous measurement of HCV NS3/4a activity. This FRET substrate is cleaved specifically by HCV NS3/4a protease thereby liberating the C-terminal peptide-fluorophore fragment from the proximity quenching effect of the dark quencher, resulting in a more than 10-fold increase in fluorescence. This assay can detect HCV NS3/4a protease activity at 1 pmole. The kit contains: • EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nm upon cleavage) • Assay buffer • Fluorescence reference standard for calibration • A detailed protocol

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Park, HR. et al. (2011). 2-arylmethylaminomethyl-5,6-dihydroxychromone derivatives with selective anti-HCV activity. Bioorg Med Chem Lett 10.1016/j.bmcl.2011.04.055.

Peng, LF. et al. (2011). Ceestatin, a novel small molecule inhibitor of hepatitis C virus replication, inhibits 3-hydroxy-3-methylglutaryl-coenzyme A synthase. J Infect Dis 204, 609.

Frick, DN. et al. (2010). A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities. Methods Mol Biol 587, 223.
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