SensoLyte ® 440 Cathepsin S Assay Kit *Fluorimetric*
Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases.
The SensoLyte® 440 Cathepsin S Assay Kit uses a fluorogenic peptide for measurement of enzyme activity. This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin S cleavage, and which can be detected with excitation at 354 nm and emission at 442 nm.
The kit contains: • AMC based fluorogenic substrate (Ex/Em=354 nm/442 nm upon cleavage) • Assay buffer • Human spleen Cathepsin S • Inhibitor • Fluorescence reference standard for calibration • A detailed protocol