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Antibodies > Primary Antibodies > Signal Transduction >> Anti-beta-Actin (NT) Antibody, polyclonal

Product Name	Anti - beta - Actin (NT) Antibody, polyclonal
Size	50 µg
Catalog #	AS-54591
US$	$209
Description	Host: Rabbit Species Reactivity: Human, mouse, rat Application: WB, IHC, ELISA Product Description: Anti-beta-Actin (NT) rabbit polyclonal antibody is supplied as an epitope-affinity purified rabbit IgG 50 µg in 250 µl (0.2 mg/ml) of 1x PBS (pH 7.4) containing 0.05% sodium azide. Immunogen: Rabbit polyclonal anti-beta-actin (NT) was raised against a 21 amino acid peptide from near the N-terminus (NT) of human beta-actin (Genbank accession NP_001092.1). Application Notes: The following concentration ranges are recommended starting points. Optimal working concentration for specific application should be determined by the investigator. ELISA for immunizing peptide: 1: 5,000-20,000 Western blot: 1: 500-1,000 Figure 1. IHC of β-actin in HeLa cells stained with rabbit polyclonal Anti-beta-Actin (NT) antibody (Cat# 54591) at 1 µg/ml. Figure 2. Western blot of β-actin in 3T3 cell lysate probed with rabbit polyclonal Anti-beta-Actin (NT) antibody (Cat# 54591). An immunoreactive band at ~42 kDa was detected (Lane A); the band was blocked by the immunizing peptide (Lane B). Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor 750-labeled secondary antibody (Cat# 61056-05-H750 or 61056-1-H750) was used and the signals detected using an infrared-imaging system (Odyssey, Li-Cor). Figure 3. Western blot of β-actin in 3T3 cell lysate (Lane A) and mouse heart tissue lysate (Lane B) probed with rabbit polyclonal Anti-beta-Actin (NT) antibody (Cat# 54591). Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor 750-labeled secondary antibody (Cat# 61056-05-H750 or 61056-1-H750) was used and the signals detected using an infrared-imaging system (Odyssey, Li-Cor).
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Detailed Information	Datasheet Material Safety Data Sheets (MSDS)
Storage	4°C
Product Citations	Mondal, D. et al. (2013). Fabrication and characterization of ZrO2–CaO–P2O5–Na2O–SiO2 bioactive glass ceramics. J Mat Sci 48, 1863. doi: 10.1007/s10853-012-6956-3. Thompson, AD. et al. (2012). Analysis of the Tau-associated proteome reveals that exchange of Hsp70 for Hsp90 is involved in Tau degradation. ACS Chem Biol 7, 1677. doi: 10.1021/cb3002599. Mizukami, C. et al. (2010). Gene silencing in Echinococcus multilocularis protoscoleces using RNA interference. Parasitol Intl 59, 647.

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