Product Description: Anti-beta-Actin (NT) rabbit polyclonal antibody is supplied as an epitope-affinity purified rabbit IgG 50 µg in 250 µl (0.2 mg/ml) of 1x PBS (pH 7.4) containing 0.05% sodium azide.
Immunogen: Rabbit polyclonal anti-beta-actin (NT) was raised against a 21 amino acid peptide from near the N-terminus (NT) of human beta-actin (Genbank accession NP_001092.1).
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentration for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1: 5,000-20,000 Western blot: 1: 500-1,000
Figure 1. IHC of β-actin in HeLa cells stained with rabbit polyclonal Anti-beta-Actin (NT) antibody (Cat# 54591) at 1 µg/ml.