The sequence (Accession # NP_003076)) corresponding to the human β-synuclein along with a 6x His tag was expressed in E. coli.
Endotoxin (EU/μg): Less than 1 EU per 1 μg of the protein as determined by Limulus Amebocyte Lysate (LAL) quantitative kinetic assay.
Beta-synuclein belongs to the family of highly conservative proteins in vertebrates. N-terminal of β-synuclein is highly homologous to α-, γ-synucleins and consists of degenerative “KTKEGV” repeats (1-4). Similar to α-synuclein, beta-synuclein is found primarily in the brain; however, it does not associate with Lewy bodies in Parkinson disease like α-synuclein (1-4). Beta-synuclein was found to inhibit production of phosphatidic acid by the phospholipase D2 transmembrane protein in vitro (1). In addition, beta-synuclein was detected in many breast and ovarian tumors (4). Recent investigations demonstrated that beta-synuclein can induce mild experimental autoimmune encephalomyelitis (EAE) in Lewis rats (5).
Purified human β-synuclein is supplied as sterile and frozen at 1 mg/ml in the following buffer: 20 mM Tris-HCl, 0.1 M NaCl, 1 mM MgCl2, pH 7.5. Store at 2-4 ºC for immediate use within 1 week or at –80 ºC for up to 6 months. Avoid repeated freeze-thaw cycles.
1. Rivers R.C. et. al. (2008) Protein Science 17: 887-898 2. Sung Y.-H. et. al. (2006) Protein Science 15: 1162-1174 3. George J. M. (2001) Genome Biology 3 (1): 3002.1-3002.6 4. Bruening W. et. al. (2000) American Cancer Society 88 (9): 2154-216 5. Kela-Madar N. et. al. (2009) Journal of Neuroimmunology 208: 19-29