SensoLyteŽ Anti-PLP (178-191) IgG Quantitative ELISA Kit (Mouse) is optimized to detect mouse anti-PLP (178-191) IgG. This kit is useful to researchers who wish to determine the amount of anti-PLP (178-191) antibody present in biological samples, and can help provide information on the role it plays in the development and treatment of EAE, an animal model for MS pathogenesis. Wells are pre-coated with PLP (178-191) peptide and pre-blocked with BSA. The amount of anti-PLP (178-191) IgG in mouse serum or cerebrospinal fluid is quantified using ELISA (Abs=450 nm).
Ample materials and reagents are provided to perform 96 assays.
* Pre-coated and pre-blocked 96-well strip plate * Ready-to-use substrate solution and other assay components * 2-3 hours assay time at room temperature
Minimal Sample Size
* Requires only 0.5-1 μl of serum or cerebrospinal fluid to perform assay
* Detects as low as 100 pg anti-PLP (178-191) IgG * No cross-reactivity with anti-PLP (139-151) IgG
Broad Dynamic Range
* 8-500 ng antibody/ml serum (depending on colorimetric developing time)