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Antibodies  >  Primary Antibodies  >  Chromatin and Nuclear Signaling  >>  Monoclonal Antibody against 5-Methylcytosine
Product Name Monoclonal Antibody against 5 - Methylcytosine
Size 0.1 mg
Catalog # BI-MECY-0100
US$ $488
Description

Host: Mouse

Clone: 33D3

Isotype: IgG1λ

Concentration: 1 mg/ml

Species Reactivity: All species

Specificity: 5-mC (5-methylcytosine)

Epitope: 5-methylcytosine found in DNA of plants and vertebrates

Storage Buffer: Phosphate Buffer Saline (+0.01% thimerosal)

Storage Instructions: Store aliquoted at -20C. Avoid repeated freeze/thaw cycles

Validated Applications: These are suggested amounts/dilutions. Optimal condition should be determined by the end user.

Blotting: 1:500
Cytochemistry: 1:500
Immunohistochemistry: 1:500
Immunoprecipitation: 1:50

RELATED PRODUCTS:
Monoclonal Antibody against 5–Methylcytosine (5-mC) (available in 3 sizes – 0.1, 0.5 & 1 mg)

Monoclonal Antibody against 5-Hydroxymethylcytosine (5-hmC), clone 4D9

5-mC & 5-hmC Starter Kit: 50 ul each of 5-Methylcytosine (5-mC) mAb, clone 33D3 and 5-hydroxymethylcytosine (5-hmC) mAb, clone 4D9
Detailed Information Datasheet
Storage -20 °C
References Reynaud, C. et al. Anal. Letters 23 , 31 (1990); Sasco, A.J. et al. Cancer Letters 108 , 157 (1996); Rougier, N. et al. Genes and development 14, 2108 (1998); Habib, M. et al. Exp. Cell Res. 249, 46(1999); Weber M. et al. et al. Nature Genetics 37, 853 (2005)
Product Citations Petropoulos, S. et al. (2014). Adult Glucocorticoid Exposure Leads to Transcriptional and DNA Methylation Changes in Nuclear Steroid Receptors in the Hippocampus and Kidney of Mouse Male Offspring. Biol Reprod 90, 43. doi: 10.1095/​biolreprod.113.115899.

Okashita, N. et al. (2014). PRDM14 promotes active DNA demethylation through the Ten-eleven translocation (TET)-mediated base excision repair pathway in embryonic stem cells. Development 141, 269. doi: 10.1242/dev.0996.
     
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