HiLyte™ Fluor555-IgG conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. HiLyte™ Fluor555 is an orange flurophore with brighter fluorescence and better photostability than Cy3. The fluorescence of HiLyte™ Fluor555 is only slightly red-shifted as compared to that of Cy3 dyes, resulting in an optimal match to filters designed for Cy3 dyes. The fluorescence of HiLyte™ Fluor555 can be observed at excitation/emission wavelength of 553 nm/568 nm.The goat anti-rabbit IgG is highly adsorbed with minimal cross-reaction to human, mouse, and rat serum protein. This secondary antibody is ideal for multi-fluorescence staining in which several primary antibodies from different species are involved.