Recombinant human α - Synuclein (1 - 140), biotin labeled
Greater than 90% as determined by SDS-PAGE and mass spectrometry.
The recombinant human α-synuclein (1-140) (GenBank Accession # NP_000336) was expressed and purified from E. coli and conjugated with biotin. Recombinant protein is produced without an affinity tag.
Parkinson's disease is predominantly a movement disorder resulting from degeneration of dopaminergic neurons in the substantia nigra. The cause of the disease is unknown, but substantial evidence suggests that the aggregation of α-synuclein is a critical step in the etiology of Parkinson's disease (PD). α-Synuclein is an abundant brain protein of 140 residues that is present in high concentrations at presynaptic terminals and is found in both soluble and membrane-associated fractions of the brain. Several possible functions have been suggested, and it appears to be involved in vesicle release and trafficking. In vitro incubation of α-synuclein in the presence of certain amounts of salt (i.e. 0.1M NaCl) with agitation can form fibril structure.
Biotinylated human α -synuclein is supplied frozen at 1 mg/ml in 10 mM sodium phosphate buffer (pH=7.4). Store at 2-4 şC for immediate use within 1 week or at -80 şC for up to 12 months. Keep in dark and avoid repeated freeze-thaw cycles.
1. Trojanowski, J. Q. & Lee, V. M. (2003) Ann. N. Y. Acad. Sci. 991, 107-110. 2. Masliah, E., et al. (2000) Science 287, 1265-1269. 3. Van Der, P. H, et al. (2000) J. Neurosci. 20, 6021-6029. 4. Feany, M. B. & Bender, W. W. (2000) Nature 404, 394-398. 5. Weinreb, P. H., et al. (1996) Biochemistry 35, 13709-13715