Existing Account

Please login first to complete purchase/ quotation request, view custom order reports, or create favorites list.

Customer ID:
Stay Logged In

Forgot your Customer ID or Password?
New Account

Don't have an account with us yet? Please set up an account to place order or obtain customer services.

Assay Kits  >  Viral/Bacterial Protease Assay Kits - HIV, HCV, TEV, WNV, Sortase, Furin  >>  SensoLyte ® 520 Sortase A Activity Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 Sortase A Activity Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72228
US$ $501

Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria. Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria. Since Sortases are widely distributed among a variety of bacterial pathogens and required for virulence, they represent a promising therapeutic target for the development of novel anti-infective agents. In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation. The SensoLyte® 520 Sortase A Activity Assay Kit is optimized for detecting Sortase A activity. This kit contains a novel internally quenched 5-FAM/QXL® FRET substrate. Sortase A cleaves the FRET substrate in two separate fragments resulting in release of 5-FAM fluorescence, which can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assays are performed in a convenient 96-well microplate format and can detect as low as 20 ng/mL active Sortase A. Kit size: 100 assays

Related Product: Sortase A Protease, Recombinant, Cat# 72229

Figure 1. Sensitivity of the SensoLyte® 520 Sortase A Assay Kit was tested using serial dilutions of recombinant Sortase A. Assay can detect as low as 20 ng/mL of active Sortase A.

Images SensoLyte ® 520 Sortase A Activity Assay Kit *Fluorimetric*
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C
  < Back