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Assay Kits  >  Amyloid Degrading Proteases Assay Kits  >>  SensoLyte ® 520 IDE Activity Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 IDE Activity Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72231
US$ $501

Insulin degrading enzyme (IDE) is considered a physiological and pathological relevant enzyme. Growing evidence has indicated that IDE may be involved in the pathogenesis of Alzheimers disease and diabetes mellitus type 2.

The SensoLyte 520 IDE Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. The unique FRET substrate was derived from an APP sequence designed to reduce cross reactivity with Neprilysin, ADAM10, TACE, BACE-1, and BACE-2. When active IDE cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence, which is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.8 ng/mL active IDE.

The kit contains:
5-FAM/QXL520 IDE substrate, Ex/Em=490/520 nm upon cleavage
Assay buffer
Recombinant human IDE
Fluorescence reference standard for calibration
A detailed protocol

Kit size: 100 assays (96-well plate)


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Figure 1. Inhibition of IDE activity by 1, 10-Phenanthroline as measured with SensoLyte 520 IDE Activity Assay Kit.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Kurauti, MA et al. (2016). Acute exercise restores insulin clearance in diet-induced obese mice. J Endocrinol. 229(3):221 doi: 10.1530/JOE-15-0483

Protzek, AO et al. (2016). Hyperinsulinemia caused by dexamethasone treatment is associated with reduced insulin clearance and lower hepatic activity of insulin-degrading enzyme. J Steroid Biochem Mol Biol. 155(Pt A):1 doi: 10.1016/j.jsbmb.2015.09.020

Boyoung, C. et al. (2016). Piceatannol, a natural analogue of resveratrol, effectively reduces beta-amyloid levels via activation of alpha-secretase and matrix metalloproteinase-9. J Func Foods. 23, 124 doi: 10.1016/j.jff.2016.02.024

Wenzhong, Y. et al. (2014). Effect of trichostatin A on gelsolin levels, proteolysis of amyloid precursor protein, and amyloid beta-protein load in the brain of transgenic mouse model of Alzheimer's disease. Curr Alzheimer Res. 11(10):1002
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