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Assay Kits  >  Alzheimer's and Parkinson's Disease  >>  SensoLyte ® Green Pin1 Assay Kit *Fluorimetric*

Product Name SensoLyte ® Green Pin1 Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72240
US$ $501
Description

Peptidyl-prolyl isomerase (PPIase) Pin1 catalyzes cis/trans isomerization of the phospho-Serine/Threonine-Proline peptide bonds (1-5). Pin1 consists of short N-terminal protein-protein interaction domain that allows enzyme to bind phosphoproteins and longer C-terminal izomerase domain (1-5). Pin1 has many biological substrates, plays critical role in cell-cycle regulation, and up-regulated in many human cancers (1-5). Recently, Pin1 was linked to the Alzheimer’s disease pathogenesis (1, 2, 4). Pin1 can bind phosphorylated Thr-212/231 residues of Tau protein and increase its deposphorylation that may prevent formation of Tau neurofibrilary tangles (1, 2, 4).

The SensoLyte® Green Pin1 Assay Kit uses a fluorogenic substrate in the cis isoform. Pin1 changes substrate into trans conformation, which is then readily cleaved to generate fluorescent signal which can be monitored at Ex/Em=490/520nm. Increase in fluorescence intensity is directly proportional to the Pin1 activity.

Figure. (A). SensoLyte® Green Pin1 Activity example (uncatalyzed corresponds to the Pin1 developer only reaction). (B). Tannic acid inhibition of Pin1 activity measured with SensoLyte® Green Pin1 Activity Assay Kit.

Images SensoLyte ® Green Pin1 Assay Kit *Fluorimetric*
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Poster
References 1. Kimura, T. et.al. J Biol Chem 288, 11 (2013): 7968-7977.
2. Galas, M-C., et.al. J Biol Chem 281, 28 (2006): 19296-10394.
3. Moore, J.D. et.al. Biorg Med Chem Lett 23 (2013): 4283-4291.
4. Dong, L., et.al. Biorg Med Chem Lett 20 (2010): 2210-2214.
5. Urusova, D.V. et.al. Cancer Prev Res 4, 9 (2011): 1366-1377.
     
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