The SensoLyte® 520 Acetylcholinesterase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of AChE inhibitors. Active AChE generates fluorescence that can be monitored at excitation/emission=490/520 nm. The long wavelength fluorescence of the substrate in this kit minimizes interference from autofluorescence of components in biological samples and test compounds, resulting in higher sensitivity.
• Optimized to detect human AChE
• Detect as low as 8 pg/ml of active AChE
• 200 assays in 96-well format
• Entire process can be completed in 1 hour
Acetylcholinesterase (AChE) is a fast acting serine hydrolase that belongs to the carboxylesterase enzyme family. It hydrolyses acetylcholine into choline and acetic acid and is found in the synaptic cleft. It can be detected in many mammals and non-mammalian species. Alzheimer and myasthenia gravis diseases are characterized by reduced acetylcholine. Thus, drugs that inhibit AChE were developed to reduce disease symptoms. AChE can be inhibited by many natural substances such as alkaloids and synthetic chemicals such as organophosphorous compounds that are frequently used as pesticides. Recently, it was demonstrated that natural compounds have variable inhibitory effect on AChE depending on the species. *For Research Use Only*