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Assay Kits  >  Alzheimer's and Parkinson's Disease  >>  SensoLyte ® 520 ECEs Activity Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 ECEs Activity Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72243
US$ $501
Description

ECEs or Endothelin converting enzymes (ECE-1/ECE-2) are known as rate-limiting enzymes for the formation of the vasoconstrictor peptide endothelin-1 (ET-1) from its precursor proendothelin-1. ECE-1 can also degrade amyloid ß fragments both in vitro and in vivo. Highly homologous and structurally comparable to ECE-1, ECE-2 was shown in homozygous knockout mice to have decreased Aß degradation and significant elevated Aß 1-40 and Aß 1-42 in the brain. The SensoLyte® 520 ECEs Activity Assay Kit can be used to detect ECE activity in biological samples or in purified enzyme preparations. The substrate provided in this kit was based on a beta amyloid fragment. When active ECEs cleave the FRET substrate, it results in an increase of 5-FAM fluorescence, and is monitored at excitation/emission = 490/520 nm. The long wavelength fluorescence of 5-FAM shows less interference from autofluorescence of components in biological samples and test compounds. This assay can detect as low as 15.6 ng/mL active ECE-1 and 62.5 ng/mL active ECE-2.

The kit contains: 

  • 5-FAM/QXL® 520 ECEs substrate, Ex/Em=490/520 nm upon cleavage 
  • Assay buffer 
  • Human recombinant ECE-1 
  • Inhibitor 
  • Fluorescence reference standard for calibration 
  • Detailed protocol 
  • Kit size: 100 assays (96-well plate not included)

    Benefits: 
  • Industry's First available kit for ECEs Activity
  • Long wavelength fluorimetric kit means lower background, higher sensitivity
  • Direct detection of both ECE-1 and ECE-2 activity
  • View our other Amyloid Degrading Assay kits
  • View our other Endothelin-1 (ET-1) Products


  • Figure 1. Inhibition of ECEs activity by Phosphoramidon as measured with SensoLyte® 520 ECEs Activity Assay Kit.
    Images SensoLyte ® 520 ECEs Activity Assay Kit *Fluorimetric*
    Detailed Information Datasheet
    Material Safety Data Sheets (MSDS)
         
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