The HA.11 antibody recognizes the influenza hemagglutinin (HA) epitope (YPYDVPDYA), corresponding to amino acids 98-106 of the human influenza hemagglutinin surface glycoprotein. This sequence has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of HA-tagged proteins. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. This affinity matrix-bound Ab can be used for immunopurification of HA-tagged proteins.
Host:MouseClone: 16B12Isotype: IgG1,kReactivity: HA.11 Tag (YPYDVPDYA)Immunogen: HA.11 was raised against the peptide CYPYDVPDYASLConcentration:2 mg/mLFormulation:PBS + 0.03% Thimerosal; The Ab was purified on Protein G and subsequently immobilized on SepharoseTM Fast Flow beads (Sepharose is a trademark of Amersham Biosciences Limited)Applications:This immobilized Ab can be used for immunoprecipitation of HA-tagged proteins. Working Dilutions:The Ab/protein binding can take place in common buffers (pH 6.0 - 7.5) in the presence of salt (50-500 mM) and reasonable levels of detergent. Excess amounts of reducing agent should be avoided as it would impact the disulfide bridges between the Ab heavy and light chains.