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Antibodies  >  OptimAb Antibodies  >>  OptimAbᵀᴹ Pax-6 Polyclonal Antibody, purified

Product Name OptimAbᵀᴹ Pax - 6 Polyclonal Antibody, purified
Host: Rabbit, Polyclonal Antibody
Size 100 g/50 L
Catalog # PRB-278P-100
US$ $282
Description

Pax-6 is a transcription factor expressed during embryonic development, mainly in eyes and brain. Pax-6 has 2 DNA-binding domains, the paired domain (PD), and the paired-type homeodomain (HD). There are at least three different protein isoforms resulting from alternative spicing, Pax-6, Pax-6(5a), and Pax-6(∆PD) isoforms. Pax-6 contains a N-terminal paired domain, a linker, a homeodomain and a proline/serine/threonine (P/S/T)-rich C-terminal domain. Pax-6(5a) results from the alternative splicing of exon 5a resulting in a 14 residue insertion in the paired domain which alters the DNA binding specificity of the transcription factor. The Pax-6(∆PD) isoforms lack the paired domain. They originate from 3 start codons and have molecular masses of 43, 33, or 32kDa. This pAb is useful in studying brain, neuronal and olfactory development in higher eukaryotes.

Host:Rabbit
Clone: N/A
Isotype: N/A
Reactivity: Extensive; the antigen sequence is highly conserved among Pax-6 of various species.
Immunogen: This pAb was raised against the peptide QVPGSEPDMSQYWPRLQ, which is present in the C-terminal part of mouse Pax-6
Concentration:2 mg/mL
Formulation:PBS + 0.03% Thimerosal. The Ab was purified on Protein A
Applications:The Ab is effective in immunoblotting (WB), immunohistochemistry (IHC) and immunofluorescence (IF). Positive control: human brain, epididymis.
Working Dilutions:WB: 1/200 - 1/500 (predicted MW: 47-50 kDa)
IHC: 1/100
IF: 1/300
This pAb is useful in studying brain, neuronal and olfactory development in higher eukaryotes.

Detailed Information Datasheet
Storage 4C
References 1, Marquardt T et al. (2001) Cell 105(1): 43-55.
2, Yamamoto Y & Jeffery WR (2000) Science 289(5479): 631-633.
3, Ashery-Padan R et al. (2000) Genes Dev. 14(21): 2701-2711.
4, Osumi N et al. (1997) Development 124: 2961-2972.
5, Koroma BM et al. (1997) Invest. Ophthalmol. Vis. Sci. 38: 108-120.
6, Davis JA et al. (1996) J. Neurosci. 16: 5082-5094.
     
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