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Antibodies  >  OptimAb Antibodies  >>  OptimAbᵀᴹ Neurofilament H&M Phosphorylated , Monoclonal Antibody, purified

Product Name OptimAbᵀᴹ Neurofilament H&M Phosphorylated , Monoclonal Antibody, purified
Clone: SMI 310; Host: Mouse, Monoclonal Antibody
Size 50 g/100 l
Catalog # SMI-310P-050
US$ $288
Description

Neurofilaments are key components of the neurons cytoskeleton whose main function is to provide structural support to the axons. There are 3 major neurofilament subunits: light or lowest (NF-L), medium or middle (NF-M) and heavy or highest (NF-H).
SMI 310 reacts with a phosphorylated epitope in extensively phosphorylated NF-H and to a much lower extent NF-M in most mammalian species. Immunocytochemically, SMI 310 reacts with selected axons. The extent of reaction with axons is more restricted than that of any other anti-phosphoneurofilaments. Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 310. SMI 310 needs less phosphatase treatment of neurofilaments for abolition of
reactivity than any other antibody to phosphorylated neurofilaments, suggesting that a very extensive degree of hyperphosphorylation of neurofilaments is necessary for its reactivity. In pathological conditions, reaction with SMI 310 may be found also in neuronal cell bodies. In Alzheimer's disease, SMI 310 provides a strong reaction with extraneuronal (ghost) neurofibrillary tangles. Intraneuronal tangles remain unreactive. SMI 310 may react with an unusual and sparsely distributed form of phosphorylated neurofilaments that may be exacerbated in certain pathologic conditions. SMI 310 distinguishes sporadic inclusion-body myositis from hereditary inclusion-body myopathies.

Host:Mouse
Clone: SMI-310
Isotype: IgG1,k
Reactivity: Mammalian
Immunogen: nd
Concentration:0.5 mg/mL
Formulation:PBS pH 7.2 + 0.09% sodium azide; The Ab is purified
Applications:The Ab is effective in immunoblotting (WB), immunohistochemistry (IHC) and ELISA.
Working Dilutions:WB: 1/1.000

Detailed Information Datasheet
Storage 4C
References 1, Mirabella M et al. (1996) J. Neuropath. Exp. Neurol. 55:774.
2, Vickers JC et al. (1992) J. Neuropath. Exp. Neurol. 51: 319.
     
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