RNA Polymerase II is the central component of the transcriptional machinery. The two largest components of RNA Pol II, RPB1 and RPB2, form the enzyme active site. The mAb 8WG16 recognizes the C-terminal heptapeptide repeat (YSPTSPS) present on the largest subunit of RNA Pol II (RPB1). Clone 8WG16 has been used to investigate the role of the C-terminal domain of the 22 kDa RNA Pol II subunit in the formation of the transcriptional complex.
Host:MouseClone: 8WG16Isotype: IgG2aReactivity: Extensive (Yeast to Human)Immunogen: RNA Pol II purified from wheat germ extractConcentration:1 mg/mLFormulation:PBS pH 7.2; The Ab is purifiedApplications:The Ab is effective in immunoblotting (WB), immunofluorescence (IF) and immunoprecipitation (IP).Working Dilutions:WB: 1/500 (Predicted MW: 220 kDa) IF: 1/50 - 1/100 IP: 1/100
1, Bhaumik SR & Green MR (2001) Genes Dev. 15(15): 1935-1945.2, Patturajan M et al. (1998) J. Biol. Chem. 273(8): 4689-4694.3, Zhang J & Corden JF (1991) J. Biol. Chem. 266: 2290-2296.4, Thompson NE et al. (1989) J. Biol. Chem. 264: 11511-11520.