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Antibodies  >  OptimAb Antibodies  >>  OptimAbᵀᴹ RNA Polymerase II , Monoclonal Antibody, purified

Product Name OptimAbᵀᴹ RNA Polymerase II , Monoclonal Antibody, purified
Clone: 8WG16; Host: Mouse, Monoclonal Antibody
Size 50 g/50 L
Catalog # MMS-126P-050
US$ $221
Description

RNA Polymerase II is the central component of the transcriptional machinery. The two largest components of RNA Pol II, RPB1 and RPB2, form the enzyme active site. The mAb 8WG16 recognizes the C-terminal heptapeptide repeat (YSPTSPS) present on the largest subunit of RNA Pol II (RPB1). Clone 8WG16 has been used to investigate the role of the C-terminal domain of the 22 kDa RNA Pol II subunit in the formation of the transcriptional complex.

Host:Mouse
Clone: 8WG16
Isotype: IgG2a
Reactivity: Extensive (Yeast to Human)
Immunogen: RNA Pol II purified from wheat germ extract
Concentration:1 mg/mL
Formulation:PBS pH 7.2; The Ab is purified
Applications:The Ab is effective in immunoblotting (WB), immunofluorescence (IF) and immunoprecipitation (IP).
Working Dilutions:WB: 1/500 (Predicted MW: 220 kDa)
IF: 1/50 - 1/100
IP: 1/100

Detailed Information Datasheet
Storage 4C
References 1, Bhaumik SR & Green MR (2001) Genes Dev. 15(15): 1935-1945.
2, Patturajan M et al. (1998) J. Biol. Chem. 273(8): 4689-4694.
3, Zhang J & Corden JF (1991) J. Biol. Chem. 266: 2290-2296.
4, Thompson NE et al. (1989) J. Biol. Chem. 264: 11511-11520.
     
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