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Antibodies  >  OptimAb Antibodies  >>  OptimAbᵀᴹ Prion , Monoclonal Antibody, purified

Product Name OptimAbᵀᴹ Prion , Monoclonal Antibody, purified
Clone: 3F4; Host: Mouse, Monoclonal Antibody
Size 100 µg/50 µL
Catalog # SIG-39620-100
US$ $361
Description

Prion protein (PrP), also known as CD230, is mainly produced in the nervous sytem. PrP exists as different isoforms, among which the normal PrPc, and the 'scrapie' isoform PrPSc. PrPSc is misfolded and associated to multiple disorders including bovine spongiform encephalopathy, Gerstmann-Straüssler-Scheindker disease (GSS) and the Creutzfeldt-Jakob disease. It contains a much higher beta-sheet content than PrPc, and tends to form protease-resistant aggregates. A hypothesis to support the propoagation of these aggregates and their role in neurodegeneration is that the change from normal PrPc is due to its interaction with PrPSc (conformation conversion hypothesis).
3F4 is reactive to amino acids 109-112 of prion protein (PrP) from humans, hamsters, and felines. It recognizes both protease sensitive and protease resistant forms of PrP (after denaturation).

Host:Mouse
Clone: 3F4
Isotype: IgG2a
Reactivity: Human, Hamster, Feline
Immunogen: nd
Concentration:2 mg/mL
Formulation:PBS (with no preservatives). The Ab is purified
Applications:The Ab is effective in immunoblotting (WB), immunohistochemistry (IHC) on unfixed tissues, immunoprecipitation (IP) and ELISA.
Working Dilutions:WB: 1/10.000 - 1/100.000
IHC: 1/100 - 1/1.000 (epitope must be unmasked by pretreatment with either 70% formic acid for 10-30min at RT, or hydrolytic autoclaving)
IP: 1/10 - 1/100
ELISA: 1/100 .000- 1/1.000.000
Strage Instruction:Store at 4°C; do not store antibody diluted below 50µg/ml in the absence of carrier protein (e.g. add 2% BSA)

Detailed Information Datasheet
Storage 4°C
References 1, Kondoh G, et al. (2005) Nat. Med. 11(2): 160-166.
2, Gilch S, et al. (2004) Traffic 5: 300-313.
3, Nishina K, et al. (2004) Biochem. 43: 2613-2621.
4, Shiga Y, et al. (2004) Neurol. 63: 443-449.
5, Zou W, et al. (2004) Proc. Natl. Acad. Sci. USA 101(5): 1380-1385.
6, Kitamoto T, et al. (1987) Lab. Invest. 57: 230-236.
7, Kascsak, et al. (1987) J. Virol. 61: 3688-3693.
     
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