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Antibodies  >  OptimAb Antibodies  >>  OptimAbᵀᴹ Ubiquitin , Monoclonal Antibody, purified

Product Name OptimAbᵀᴹ Ubiquitin , Monoclonal Antibody, purified
Clone: P4D1; Host: Mouse, Monoclonal Antibody
Size 50 g/100 l
Catalog # MMS-257S-050
US$ $178
Description

Ubiquitin is a highly-conserved small protein expressed in all eukaryotes. It is involved in a post-translation modification of proteins called ubiquitination of proteins by the covalent attachment (via an isopeptide bond) of one or more ubiquitin monomers. Ubiquitination controls the protein degradation, stability and intracellular localization of many proteins. PAD1 recognizes ubiquitin, polyubiquitin and ubiquitin-conjugated proteins. Ubiquitin is a well-conserved protein, hence P4D1 has extensive species cross-reactivity. PAD1 was developed to detect ubiquitin and ubiquitin-substrate conjugates by western blotting. Because the Ab recognizes a denatured epitope, it is known not to work in immunoprecipitation or immunostaining applications.

Host:Mouse
Clone: PAD1
Isotype: IgG1,k
Reactivity: All species (yest to human)
Immunogen: This Ab was raised against denatured bovine ubiquitin
Concentration:0.5 mg/mL
Formulation:PBS pH 7.2 + 0.09% sodium azide; The Ab is purified
Applications:The Ab is effective in immunoblotting (WB) and ELISA.
Working Dilutions:WB: 1/1.000 (predicted MW = 9 kDa)

Detailed Information Datasheet
Storage 4C
References 1, Kahana A & Gottschling DE (1999) Mol. Cell; Biol. 19(10) 6608-6620.
2, Hochstrasser M (1996) Annu. Rev. Genet. 30, 405-439.
     
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