Ubiquitin is a highly-conserved small protein expressed in all eukaryotes. It is involved in a post-translation modification of proteins called ubiquitination of proteins by the covalent attachment (via an isopeptide bond) of one or more ubiquitin monomers. Ubiquitination controls the protein degradation, stability and intracellular localization of many proteins. PAD1 recognizes ubiquitin, polyubiquitin and ubiquitin-conjugated proteins. Ubiquitin is a well-conserved protein, hence P4D1 has extensive species cross-reactivity. PAD1 was developed to detect ubiquitin and ubiquitin-substrate conjugates by western blotting. Because the Ab recognizes a denatured epitope, it is known not to work in immunoprecipitation or immunostaining applications.
Host:MouseClone: PAD1Isotype: IgG1,kReactivity: All species (yest to human)Immunogen: This Ab was raised against denatured bovine ubiquitinConcentration:0.5 mg/mLFormulation:PBS pH 7.2 + 0.09% sodium azide; The Ab is purifiedApplications:The Ab is effective in immunoblotting (WB) and ELISA.Working Dilutions:WB: 1/1.000 (predicted MW = 9 kDa)