Paired box (Pax) proteins are tissue-specific transcription factors that contain a paired domain and a homeodomain. They are produced during embryogenesis in a temporally and spatially restricted manner in the developing neural tube. Pax-2 has been associated to kidney and optic nerve development. This antibody recognizes Pax-2A and Pax-2B (two products of the Pax-2 gene). When conjugated to agarose beads, this Ab has been used to immunoprecipitate DNA sequences which are the target of Pax-2.
Host:RabbitClone: N/AIsotype: N/AReactivity: Human, Mouse, Chicken, Xenopus and ZebrafishImmunogen: This pAb was raised against a recombinant protein corresponding to amino acids 188-385 from Pax-2Concentration:1 mg/mLFormulation:PBS + 0.03% Thimerosal; The Ab is purifiedApplications:The Ab is effective in immunoblotting (WB), immunohistochemistry (IHC), immunoprecipitation and DNA band supershift experiments. Positive control tissues: normal kidney, renal adenocarcinoma.Working Dilutions:WB: 1/250 - 1/1.000 (predicted MW = 44 kDa) IHC: 1/100 Gel shifts: 1/10
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