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Recombinant Proteins  >  Sortase  >>  Sortase A protease, Recombinant

Product Name Sortase A protease, Recombinant
Size 10 µg
Catalog # AS-72229
US$ $374

Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria. Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria. Sortase A protease can be used with SensoLyte®520 Sortase A Activity Assay Kits (AnaSpec Cat# 72228) to screen for anti-infective agents.

Sortase A from Staphylcoccus aureus was expressed in E. coli expression system with a N-terminal His tag. It is comprised of 26-206 amino acids and its MW is 21.7-kDa. The activity of Sortase A can be measured in a FRET-based enzymatic assay, SensoLyte® 520 Sortase A Activity Assay Kit, Cat# 72228. 100 ng of enzyme is sufficient for the assay.

Sortase A is stored in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, and 2.2 mM KCl, 8 mM imidazole, 0.04% Tween 20, and 20 % glycerol.

The purity is >87% as estimated on SDS PAGE.

Related Product: SensoLyte® 520 Sortase A Activity Assay Kit *Fluorimetric*, Cat# 72228

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
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