This kit is optimized to detect rat alpha-Synuclein (α-Synuclein) in cell and tissue lysate, and in body fluids such as plasma. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.
One Step Convenient Format
Pre-coated and pre-blocked 96-well strip plate
Ready-to-use substrate solution and other assay components
One step assay (samples and detection antibody are added simultaneously)
1 hour assay time at room temperature (excluding incubation time)
Minimal Sample Size
Requires only 10-20 μl of cell and tissue lysate or body fluids
Detects 5 pg/ml of α-Synuclein (calculated as three standard deviations from the blank)
1. Rivers, R. et al. Protein Science 17, 887-898 (2008). 2. Bruening, W. et al. Cancer. 88, 9, 2154-2163 (2000). 3. George M. J. Genome Biology 3, 1, 1-6 (2001). 4. Latawiec, D. et al. PloS ONE 5, 2, 1-8 (2010).