SensoLyte ® 520 IDE Activity Assay Kit *Fluorimetric*
Insulin degrading enzyme (IDE) is considered a physiological and pathological relevant enzyme. Growing evidence has indicated that IDE may be involved in the pathogenesis of Alzheimer’s disease and diabetes mellitus type 2.
The SensoLyte® 520 IDE Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. The unique FRET substrate was derived from an APP sequence designed to reduce cross reactivity with Neprilysin, ADAM10, TACE, BACE-1, and BACE-2. When active IDE cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence, which is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.8 ng/mL active IDE.
The kit contains: • 5-FAM/QXL520® IDE substrate, Ex/Em=490/520 nm upon cleavage • Assay buffer • Recombinant human IDE • Inhibitor • Fluorescence reference standard for calibration • A detailed protocol