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Assay Kits  >  Alzheimer's and Parkinson's Disease  >>  SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*

Product Name SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*  NEW
Size 1 kit
Catalog # AS-72258
US$ $457
Description

Glucocerebrosidase/GBA (also called acid β-glucosidase, Glucosylceramidase,) is a lysosomal enzyme responsible for the breakdown of glucocerebroside releasing glucose and ceramide. Deficiency of this enzyme due to genetic mutations leads to accumulation of glucocerebroside and development of lysosomal storage disease, known as Gaucher disease (GD). Mutations in the glucocerebrosidase (GBA1) gene are also associated with increased risk for Parkinson disease and related disorders. It has been hypothesized that GBA, when not available to clear out proteins like alpha-synuclein, results in the accumulation of the proteins thereby contributing to Parkinson’s disease.
The SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a most common fluorogenic substrate, MUGlc, for measurement of GBA activity. In presence of GBA the colorless substrate is hydrolyzed into 4-methylumbelliferone (4MU), and after addition of stop solution a bright blue fluorescence can be monitored at excitation/emission = 365nm/445nm nm.

Related Product (ex/em=570nm/610nm)SensoLyte® Red Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*
Fig 1. Inhibition of GBA activity by Isofagomine as measured with SensoLyte®Blue Glucocerebrosidase Assay Kit.Fig 2. Activation of GBA activity by GCase Activator (NCGC00188758). 5ng of GBA enzyme was incubated with and without 10µM of GCase activator and fluorescence was measured with SensoLyte®Blue Glucocerebrosidase Assay Kit.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
References
  1. Xu, Y. H et al, Am.J.Pathol, 163, 2093(2003)
  2. Sidransky, E et al, N Engl J Med, 361, 1651(2009)
  3. Murphy K.E et al, Brain,137, 834(2014)
  4. Sybertz, E. et al, J Lipid Res 55, 1996 (2014)
     
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