Kaneka endonuclease is a genetically engineered endonuclease. It efficiently cleaves A variety of DNA and RNA into small fragments, irrespective of being single-stranded, double-stranded, linear, circular or supercoiled. The enzyme can be used for various applications, such as removal of DNA/RNA from solutions, reduction of cell lysate viscosity caused by nucleic acids, and so on. This Endonuclease is a homodimer of 27 kDa subunit with a calculated pI of pH6.6, and Mg2+ is required for enzyme activity.
KanekaEndonuclease
Specification
Specification Value
Appearance
Colorless, Clear Transparent
Activity
> 250 U/µl
Specific Activity
> 6.0 x 105 U/mg
Endotoxin
< 0.25 EU/kU
Protease
Not Detectable
Total Microbial Count
< 10 CFU in 100 kU
Supplied in 50%(v/v) glycerol, 20 mM Tris-HCl (pH 8.0), 2 mM MgCl2, and 20 mM NaCl.
Datasheet
